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KMID : 0613820070170030340
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Journal of Life Science
2007 Volume.17 No. 3 p.340 ~ p.344
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Inactivation of Avian Influenza Viruses by Alkaline Disinfectant Solution
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Seo Sang-Heui
Lee Joo-Seob
Lee Chang-Jun
Kim Heui-Man
Jo Su-Kyung
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Abstract
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Avian influenza viruses cause a considerable threat to humans and animals. In this study, we investigated whether alkaline disinfectant solution can inactivate H5N1, H3N2, H6N1, and H9N2 subtypes of avian influenza virus. When H5N1, H3N2, H6N1, and H9N2 avian influenza viruses were treated with alkaline solution diluted with PBS (pH 7.2) prior to infection into MDCK cells, alkaline disinfectant solution (at dilutions up to 10-2) completely inactivated all avian influenza subtypes tested. To confirm the inactivation of avian influenza viruses by alkaline disinfectant solution, we used an immunofluorescence assay with influenza A anti-nucleoprotein antibody and FITC-labeled secondary antibody to stain MDCK cells infected with avian H9N2 influenza viruses. No staining was observed in MDCK cells infected with H9N2 viruses that were pre-treated with a 10-2 dilution of alkaline disinfectant solution, while strong staining was observed in MDCK cells infected with H9N2 viruses without pre-treatment. Our results indicate that alkaline solution could help to control avian influenza viruses including the highly pathogenic H5N1 subtype.
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KEYWORD
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Avian influenza virus, H9N2, H5N1, H6N1, H3N2
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